The 2000 Preston by-election was a parliamentary by-election held in England on 23 November 2000, to elect a new Member of Parliament (MP) for the House of Commons constituency of Preston in Lancashire.
The vacancy was caused by the death on 2 September 2000 of Audrey Wise, the constituency's Labour Party MP. The Labour vote share declined, but with the main beneficiaries being fringe parties, the Labour candidate Mark Hendrick held the seat comfortably. The by-election was held on the same day as the 2000 West Bromwich West by-election and the 2000 Glasgow Anniesland by-election, both also won by the Labour Party.
Peter Garrett used the description "Preston Christian Alliance". David Braid used the description "Battle for Britain".
23 November 2000 was the last time three or more by-elections occurred on a single day until November 2012, when three by elections occurred on 15 November and three more on 29 November.
Results.
! scope="col" colspan="2" style="width: 130px"|Party
! scope="col" style="width: 17em"|Candidate
! scope="col" style="width: 5em"|Votes
! scope="col" style="width: 3.5em"|%
! scope="col" style="width: 3.5em"| ;templatestyles src="Template:Tooltip/styles.css" / ;±%
! colspan="3" style="text-align: right; margin-right: 0.5em" | Majority
! colspan="3" style="text-align: right; margin-right: 0.5em" scope=row | Turnout
! style="text-align:right;" | Swing
Previous general election result, 1997.
! scope="col" colspan="2" style="width: 130px"|Party
! scope="col" style="width: 17em"|Candidate
! scope="col" style="width: 5em"|Votes
! scope="col" style="width: 3.5em"|%
! scope="col" style="width: 3.5em"| ;templatestyles src="Template:Tooltip/styles.css" / ;±%
! colspan="3" style="text-align: right; margin-right: 0.5em" | Majority
! colspan="3" style="text-align: right; margin-right: 0.5em" scope=row | Turnout
! style="text-align:right;" | Swing
References.
 ;templatestyles src="Reflist/styles.css" / ;