The women's 100 metres hurdles event at the 2009 Summer Universiade was held on 9–10 July.
Results.
Heats.
Qualification: First 3 of each heat (Q) and the next 4 fastest (q) qualified for the semifinals.
Wind: ;br ;Heat 1: 0.0 m/s, Heat 2: +1.6 m/s, Heat 3: +0.4 m/s, Heat 4: +0.6 m/s
Semifinals.
Qualification: First 3 of each semifinal (Q) and the next 2 fastest (q) qualified for the finals.
Wind: ;br ;Heat 1: +0.5 m/s, Heat 2: −0.3 m/s
Final.
Wind: +1.5 m/s