Amersham Museum at 49 and 51 High Street is a small local museum based in Amersham, Buckinghamshire, England. It is located on the High Street in Old Amersham.
The museum is partly located in a 15th-century half-timbered house, listed Grade II on the National Heritage List for England, with a herb garden to the rear, and covers local history. It has collections of fossils and archaeological finds, including objects from Roman and mediaeval times, and displays on local crafts including chairmaking, lace making and straw plait. It originally opened to the public in 1991. In 1993 the museum won a National Heritage Award. In 2001 it was extended at the front of the museum, and in 2005 it employed its first professional curator. 
In 2017 the Museum re-opened following a major development that included expansion into the neighbouring property at 51 High Street, a former post office. This enabled the museum to create a new introductory exhibition gallery, combined activity and temporary exhibition space, two collections stores, indoor adapted WC and lift. The museum also gained a modern glass reception and shop.
In March 2024 the Museum re-opened with a new temporary exhibition gallery, the Marie-Louise von Motesiczky Gallery, named for the emigree Austrian artist who lived in Amersham during the Second World War, and whose Charitable Trust funded the redevelopment. 
As of  2023[ [update]], the museum is open from 12noon to 4.30pm from Wednesdays to Sundays plus Bank Holiday Mondays. Normal admission is £6 for adults (£7 including donation) children free. An annual pass, allowing access for a full year, is £10.
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