If you are content with 10k or so survivors, never!
Procedure is as follows:
day 1: Space station is declared quarantine. All frozen embryos are declared quarantine, along with pre-existing frozen eggs and sperm.
year 1: Space station is being resupplied by sterilized food packages. Development in automation commences.
year 2: controlled breeding program established in space station (we are at serious risk of inbreeding now but it won't matter too much). The population growth has to be carefully controlled here, as a large population would be fatal to further plans.
year 3: sterilized nursery delivered to space station
year 10: beginning to divert resources from education to production
year 15: large hab modules delivered to space station
year 20: all further development proceeds on automation and AI platform
year 25: college & electronic library delivered to space station
year 30: mass rollout of solar technology
year 40: collection of internet library; space station supplies should be extended to +50 years by this point, space station boosted to +50 year orbit. Elderly care starts consuming economy
year 50: delivery of 100 long-lasting re-entry capsules to space station. Space station supplied to +70 years, including 3d printers and raw materials. Further resupplies about to cease.
year 51: collection of knowledge in libraries and long-lasting electronics should be completed. 335
Elderly care overruns economy.
year 52: Space station is on its own.
year 60: Mass die-offs begin due to inadequate medical care
year 70: Embryo storage power supply is converted to RTGs.
year 80: Runaway mass die-offs has reduce quality of life to terrible levels.
year 120: Humans extinct on earth by this point. (Yes we have a few 120+ alive now, but without support they won't be). The disease is extinct with them.
year 121: The sixth generation returns from the space station. They can tap the remains of the automated systems to begin farming. Female population: 100
year 122: Crack open the first of the frozen embryos.
year 142: At maximum rate, female population: 500 (200 adults). This assumes that automation development worked and we can sustain this population boom. Once reaching this stage though, it's no longer a problem.
year 162: Runaway population boom subsides due to running out of frozen embryos. This brave new world has very few human-specific pathogens. 998