Statistical analysis will include the chi-square, Fisher exact test, t test and paired t test. The chi-square will be used to test the difference in proportions. Fisher exact test will be for whatever the expected value is less than 5; paired t test will used for comparing the pre-and post-treatment results. For comparison of mean between groups, t test will be done. All the data will be presented as mean ± SEM, and P < 0.05 will be considered statistically significant in all cases.