Each slide containing cytokine standards used for making known serial dilutions, with sample diluent serving as the negative control, and experimental samples will then be incubated overnight at 4°C followed by washing unbound materials. The detection antibody will then bound to the antigens within each well. Cy3 equivalent dye-conjugated streptavidin will be pipetted in each well, which binds to the detection antibody associated with immune complexes. The slides will be incubated and covered with aluminum foil to avoid light exposure and the fluorescence from each well was detected using a laser scanner.¶ The resultant signals of the samples will be compared to the standard curve for each of the cytokines in order to determine the concentrations of each cytokine within the samples. Data will be extracted and analyzed using microarray analysis software.#