Total abundance of GSH/GSSG will be measured using commercial kits (Cayman Chemical, Ann Arbor, MI). MnSOD and CuZnSOD will be evaluated using an activity assays. Notably, mice lacking CuZnSOD are a model for age-related muscle loss and exhibit increased production of reactive oxygen species. Likewise, MnSOD activity has been suggested to play a role in age-related muscle loss and dysfunction.