After the second biopsy, subjects will consume a test meal, with 10 grams of leucine, within 10 minutes. To avoid disruption of the phenylalanine isotopic steady state due to ingestion of unlabeled amino acids, L-[ring-13C6]Phenylalanine, [methyl d3] 3-methylhistidine and [methyl d3] methionine will be added to the meal to enrich it to the expected arterial enrichments. The investigators have shown that this approach can adequately prevent changes in the arterial isotopic enrichment during bolus ingestion of an amino acid supplement, thus allowing for steady state equations to calculate muscle protein synthesis. After meal intake the investigators will follow the blood amino acid concentrations and enrichments with frequent blood sampling. At t=6 and t=7 a third and fourth muscle biopsy will be taken to measure muscle protein synthesis, cell signaling, and redox homeostasis during treatment and in response to the meal. Between t=6 and t=7 Doppler blood flow and muscle perfusion (CEU) will be measured again to determine the effect of treatment and meal on these parameters. After the last muscle biopsy, the tracer infusion will be stopped and the subject will be made comfortable and fed.