1. OBJECTIVES The main objective will be to identify whether a bolus (140 mL) ingestion of dietary nitrate (~8 mmol NO3-) in the form of concentrated beetroot juice will acutely increase time-trial performance compared to a nitrate-depleted placebo (140 mL; ~0.0047 mmol NO3-). A secondary objective will be to measure blood samples after nitrate and meal ingestion to assess any changes in the concentration of nitrate, nitrite, glucose, insulin, lactate and free fatty acids. In this secondary objective, we are most interested in determining whether nitrate supplementation changes blood metabolites (glucose, insulin, lactate, free fatty acids) following 1) meal ingestion and 2) immediate post-exercise recovery period in comparison to the placebo. We will test the hypothesis that a single dose (140 mL; ~8 mmol NO3-) of dietary nitrate supplementation (beetroot juice) ingested 3 h prior to exercise will improve time-trial performance in trained cyclists compared to the nitrate-depleted placebo.