The long-term goal is to provide new insight into the effect of long-lasting sequence movements (sequence effect) by investigating de novo and advanced Parkinson's disease (PD) patients with an objective measurement. Our central hypothesis is that sequence effect is caused by depressed excitability of the motor cortex, and is partially associated with dopaminergic deficiency. Specifically, the aims of this study are to show that the sequence effect is more severe in advanced PD than in de novo PD and to evaluate the extent of improvement by levodopa and repetitive transcranial magnetic stimulation (rTMS) and to show the correlation of fatigue.