200mL of abdominal adipose tissue was obtained under local anesthesia by liposuction aspirates protocol. The lipoaspirate was washed with PBS to remove tissue debris. 100mL PBS containing 0.1% w/v collagenase A type I was added to isolated tissue and then incubated at 37ºC for 60 minutes. Collagenase activity was neutralized using MEM medium along with 10% fetal bovine serum. Cell pellets were resuspended in culture medium after centrifugation at 2000rpm for 10 min, and then transferred to culture flasks for 72 h at 37ºC in 5% CO2 condition. The culture medium in the flasks was changed every 3 days, and cells were passaged for two times.