Metabolic and immune-inflammatory biochemical evaluation Will be evaluate plasma levels of ANP, BNP, Leptin, visfatin, and IL-1β, TNF-a, IL-6 that will be measured using a sandwich ELISA (Human IL-1β, TNF-a, IL-6 6 Diaclone). ANP and BNP plasma concentration was measured in duplicate by a solid phase sandwich immune-radiometric assay for human BNP (IRMA, ANP and BNP, Shering cis bio int). The minimum detectable concentrations for the diagnostic tests are: TNF-a: 8pg/mL; IL-1β: <1pg/mL; IL-6: <0.81pg/mL; ANP: 3.1pg/mL; BNP: 5pg/mL.