Patients who smoked for more than 10 years, consuming 10 more cigarettes per day and diagnosing chronic periodontitis (case) and another group (control) were non-smokers with chronic periodontitis, according to the standard of WHO definition of the smoking population,The ChP was diagnose in accordance with the recommended standards from the Center for Disease control and Prevention, the American Academy of Periodontology on the condition of patients in good general health, aged over 30 years, presenting with pocket periodontal disease with CAL ≥ 5 mm and radiographic bone loss and bleeding after probing.