Amplicon processing and 454 sequencing were conducted at the Lund University Sequencing Facility (Faculty of Science, Lund, Sweden). After amplicon cleaning to remove short fragments (Agencourt AMPure XP; Beckman Coulter, Brea, CA, USA) and inspection (DNA 1000 kit on a 2100 Bioanalyzer; Agilent Technologies, Palo Alto, CA, USA), amplicons were quantified (Quant-iT ds DNA assay kit; Invitrogen, Carlsbad, CA, USA and Quantifluor fluorometer; Promega, Madison, WI, USA) and diluted to obtain a total of 107 copies/μL. Titration and library production (target: 10%-15% enrichment) were performed using emulsion PCR and the Lib-A kit (Roche Diagnostics, Branford, CT, USA). DNA-positive beads were enriched, counted (Innovatis CASY particle counter; Roche Innovatis, Bielefeld, Germany), processed (XLR70 sequencing kit; Roche Diagnostics), and loaded onto a picotiter plate for pyrosequencing on a 454 Life Sciences Genome Sequencer FLX+ machine (Roche Applied Sciences; Penzberg, Germany).