Studies made in a rat glioma model demonstrated that Indocyanine Green (ICG) remained within tumors with relatively few glioma cells seen beyond the ICG-laden tumor margin. The doses utilized ranged from 60 to 120 mg/kg with imaging taking place at least one hour following the dose. In Dr. Singhal's lab, ICG-injection utilizing canine tumor models demonstrated reliable uptake into flank tumor xenografts with visualization of the tumor/normal tissue interface