Samples for DNA extraction will be collected at all the specified time points as described below. Samples will be taken in the morning at least two hours after meals Collection of plaque and saliva samples will be done as described by Keijser et al (13).Samples will be taken in the morning 5 ml of saliva will be collected using cryovials (Salimetrics) by asking the subject to passively drool saliva into the device. Samples will be placed in ice immediately and stored at -800C. A sterile microbrush will be used to collect Supragingival plaque samples from the buccal dental surfaces. After sample collection, the tip of the brush will be cut and stored inside an Eppendorf tube at -800C The Supragingival plaque collection will be done for six teeth (16, 11,24,41,36, and 44).