The phase 1 trial described here will enroll healthy adults who have not been infected with or exposed to hookworm who are living in the Washington DC area. A phase 1 trial of Na-GST-1/Alhydrogel (with or without GLA-AF) is planned to start in July 2011 in Brazil. In that study, both hookworm-naïve and hookworm-exposed healthy adults will be vaccinated with either Na-GST-1/Alhydrogel®, Na-GST-1/Alhydrogel® co-administered with 1 µg GLA-AF, or the Butang® hepatitis B vaccine. The objectives of that study will be to assess the safety and immunogenicity of the Na-GST-1 formulations in healthy Brazilian adults. The study described here will complement the study in Brazilian adults as it will provide additional information about different dose concentrations of GLA-AF as well as detailed data on the humoral immune response to the different formulations that will be tested.