Sample Collection and Analysis: Breath samples will be collected using breath bags (single use collection bags, EasySampler System, QuinTron Instrument Company, Inc. Milwaukee, WI) in disposable glass Exetainer® tubes (Labco Limited, Buckinghamshire, UK) using a collection mechanism that permits removal of dead air space. The breath enrichment of 13CO2 will be measured using isotope ratio mass spectrometer (IRMS).