Sample storage and processing. Blood samples to measure amino acid enrichments and concentrations will be collected in an ice-cold sulfosalicylic acid solution containing appropriate internal standards, spun, and the supernatant stored in cryovials at -80 Celsius until further analysis. Blood samples for hormonal and cytokine assays will be collected in clotting-activated tubes, immediately spun and the supernatant serum will be stored in cryovials at -80 Celsius. Muscle samples for measurement of amino acid free enrichment and incorporation in contractile proteins, and for molecular biology assays will be immediately rinsed with ice-cold saline, blotted and quickly (less than 5 seconds) frozen in liquid nitrogen and stored in cryovials at -80 Celsius until further analyses. Immediately after collection, muscle samples for microscopic and morphologic analysis will be mounted and snap frozen at -80 Celsius until later analysis.