The breast NAF and blood will be analyzed for cholesterol using spectrophotometric kits. Fatty acids will be analyzed using gas chromatography. Total 8-isoprostanes will be analyzed by immunoassay kits. Micronutrients and vitamins will be analyzed by high pressure liquid chromatography. Nuclear morphology will be analyzed by a computer-driven microscope. DNA damage in breast cells will be analyzed using gel electrophoresis of lysed cells (comet assay). Statistical analyses will allow us to determine if the NAF and blood measures are related to each other and to the nuclear measures.