The assays will be run according to the established protocol using the 96 well plate provided. Briefly, each serum sample is to be incubated with cytokine microbeads for 1 hour at room temperature. After washing two times, the beads will be incubated with detection antibodies for 30 min. The beads will then be combined with streptavidin-phycoerythrin and remain at room temperature for an additional 30 minutes. After two additional washings, the beads will be resuspended in assay buffer for five minutes and then the beads are to be read on the Luminex Instrument.