Enhanced chemiluminescent assay (ECL) for total antioxidant capacity (TAOC) The ECL assay for total AO capacity (TAOC) was developed by our group 12 and is based upon the horse radish peroxidase catalysed oxidation of luminol by hydrogen peroxide. A BioOrbit 1250 luminometer (Labtech International, Sussex, UK), interfaced with a PC running software which allows simultaneous recording / display of up to 5000 data points over a period up to 1 hour is used to quantify light output. The enhancer, p-iodophenol, is no longer commercially available but is synthesised "in house". Antioxidant capacities of test samples will be derived from standard curves produced using the water-soluble vitamin E analogue, 6-hydroxy-2,5,7,8, tetramethylchroman-2-carboxylic acid (Trolox; assay range: 0.0625-1.6 nanomole) which will be run before and after test samples and results expressed as a concentration (µM Trolox equivalents/L; Teq/L).