Design: Normal surgical waste (bone with red marrow, dermis, spleen, thymus and adipose) from procedures that are performed on males and females at Suburban Hospital will be placed in nutrient medium (provided by NIDCR) and sent to the NIDCR for the establishment of cell culture strains. Only the age, gender and site from which the tissue was removed will be recorded. The cell cultures from the different tissues will be used to determine their phenotypic character and differentiation properties by molecular profiling, and for studies to elucidate the regulation of gene expression and protein synthesis. Similarly, a portion of the samples obtained at Suburban Hospital will be used to generate histological sections of the normal tissues that will be used to screen newly developed antibodies and molecular probes. In some cases, RNA will be extracted from the tissues for RT-PCR analysis. These samples (cells, sections, mRNA) will also serve as normal controls for studies performed on samples obtained from patients with various diseases recruited to NIH under current NIH protocols (97-D-0055, 97-D-0145, 01-D-0184).