Statistical analysis The normality of distribution of the analyzed variables was evaluated with the Shapiro-Wilk test and Kolmogorov-Smirnov test with the Lilliefors correction. The results of the tests showed that distributions of almost all parameters significantly differed from the normal distribution. Therefore, nonparametric methods were used in statistical analysis. The Wilcoxon signed-rank test was applied for the evaluation of the differences between the baseline values and those obtained after treatment. The t test was used for variables with normal distribution. Correlations between the values of the parameters were evaluated using the Spearman's rank correlation coefficient. P value of less than 0.05 was considered significant. The Statistica software, version 10, was used for the analysis (www. statsoft. com; license JGNP410B316631AR-J, Stat- Soft, Inc., 2011, Tulsa, Oklahoma, United States).