One factor that may affect the shape of nuclei in breast cells is the level of oxidative stress in the breast. The breast fluid is very rich in fats, or lipids, and certain kinds of lipids are easily oxidized. NAF also contains vitamins obtained from fruits and vegetables that function as antioxidants to stop lipid oxidation. For example, carotenoids can react with harmful oxidants to form oxidized carotenoids. We will therefore measure levels of one lipid peroxidation marker and one oxidized carotenoid in NAF as markers of oxidative stress. We also will measure fatty acid levels and levels of un-oxidized carotenoids. We then will determine if differences in the composition of the NAF are related to the measures of nuclear shape made on breast cells.