Main trials: All trials will be conducted in a randomised and counter-balanced fashion. For each visit, the participants will be required to arrive at the NIHR Clinical Research Facility laboratory on a morning (~07:00 - 09:00 AM). On the first visit, anthropometric variables will be collected (mass, stature, BMI) prior to experimentation. On each visit, the participant will assume a seated and rested position whilst an 18 gauge cannula is inserted into the antecubital vein of their non-dominant arm for collection of venous blood. This will be kept patent with periodic infusion of saline. After cannulation is complete, a 12 ml resting blood sample will be collected. Participants will then perform one of four trials: