Commercially available enzyme-linked immunosorbent assays (ELISAs) for sFlt 1 (BioSource™) (Invitrogen Corporation, Carlsbad, CA), endoglin (R&D Systems Inc, Minneapolis, MN), PIGF (R&D Systems, Inc. Minneapolis, MN) will be used according to established protocols. Briefly, various samples for ELISA measurement will be diluted in respective calibrator diluent. After adding assay diluent, the diluted sample will be placed in a 96-well plate precoated with captured antibodies directed against human sFlt-1, human endoglin, or PlGF, and the plates were incubated for 2 hours. The wells will be washed 4 times in wash buffer and incubated with secondary polyclonal antibody against sFlt-1, endoglin, and PIGF conjugated to horseradish peroxidase for an additional 2 hours. The plates are then washed 4 times in wash buffer.