Whole Saliva Collection Unstimulated whole saliva will be collected from each subject at the beginning of the appointment, as previously described by Ramseier et al. Subjects' will vigorously rinsed their mouth with tap water for 20 seconds in order to remove gross debris. The subject then expectorates the water. Following a waiting period of 2 minutes, subjects will tip their head over the graduated test tube and passively expectorated whole saliva into the plastic funnel placed inside the plastic tube (Mandel et al. 1976). Each tube will be labeled with the subject's initials, harvest date and sample name. The collection will be completed once 2mL of whole saliva is collected, or a maximum of 15 minutes of sampling time is reached. The sample will then immediately placed on ice, supplemented with a proteinase inhibitor combination of 1% aprotinin (1mg/ml) and 0.5% phenylmethylsulphonyl fluoride (PMSF) (200mM in MeOH) (Sigma Chemical Company, St-Louis, MO), and aliquotted prior to storage at -20°C until analysis.