Task2: Adipokine measurements Total RNA from adipose tissue samples will be extracted by the Trizol technique and RNA quality will be checked by gel agarose electrophoresis and quantification by NanoDrop. After reverse transcription of encoding mRNA into cDNA, control quality of reverse transcription PCR amplification will be done using a housekeeping gene in the low number of cycles and new control by gel agarose. Real-time quantitative PCR will then be performed in triplicate on nine genes of interest (adipokines) and three housekeeping genes for normalization (18S, beta-actin and SDHA), ie 10 800 points generated. PCRs will be made in 384-well plates and the reaction mixtures distributed by robot distribution.Adipokine list (n=9): chemerin, interleukin-6 (IL-6), plasminogen activator inhibitor-1 (PAI-1), retinol binding protein 4 (RBP4), tumor necrosis factor-alpha (TNF alpha), visfatin, leptin, adiponectin, apelin.