where Wmax is the maximal workload capacity determined during Visit 1 and 3,600 is the duration in seconds (equivalent to 1 hr). The ergometer will be set in linear mode to obtain 60% Wmax when subjects' cycle at their preferred cadence determined during Visit 1. The ergometer will be connected to a computer that will calculate and display the total amount of work performed. Subjects will receive no verbal or physiological feedback during the time-trial. The only information subjects will receive is the absolute amount of work performed and the percentage of total work performed relative to the set amount of work that needs to be completed. A fan will be placed 1 meter behind each participant to provide cooling and air circulation during the trials. Heart rate (Polar, Finland) will be recorded continuously throughout the test. This type of time-trial has been validated and used before in our lab (Beelen, Berghuis et al. 2009), for an overview of validation studies see (Currell and Jeukendrup 2008). Examples of coefficients of variation in similar trials are 1.1 (Palmer, Dennis et al. 1996), 0.7 (Smith, Davison et al. 2001) and 0.9 (Laursen, Shing et al. 2003). Water will be provided ad libitum during visits 1 and 2. However, the water consumed during visit 2 (familiarization trial) will be measured and repeated for visits 3 and 4 (exercise trials).