The feasibility of the selection of nTreg from human peripheral blood using the CliniMACS® System has been previously documented. In these studies, CD8+ and CD19+ cells were undetectable in the final blood products. Final content of CD4+CD25+CD127lo-expressing cells was in mean 79% (range 52-82%) with the improved CD25 enrichment procedure. This protocol has also been validated by two partner Centres in the Treg GENERATION CONSORTIUM, Liège and Boston, with results that meet the release criteria for infusion. These purification procedures provided between 0.98 and 2.93x10e6 Treg per kg. It is noteworthy that the degree of purity in terms of CD4+CD25+CD127- cells ranged between 72.80% and 93.90%.