With impression cytology, the morphology of the corneal and conjunctival epithelial cells, their staining behavior, and nuclear/cytoplasmic ratio could be observed in detail. Other important characteristics such as goblet cell density, the degree of keratinization of the epithelial cells, the quality of the cell-to-cell cohesion, and the condensation of the nuclear chromatin could also be detected through proper specimens. [3, 22-26] Furthermore, with the improvement of immunocytochemical staining and confocal microscopy, specimens of impression cytology can be further analyzed for complicated ocular disorder such as cytokeratins 3 and 19 are useful in classification of limbal stem cell deficiency [27] and mucin secretion of goblet cells can be evaluated by confocal microscopy [28].