ADMSCs were harvested at 90% confluence before injection for freezing. To collect cells, culture medium was removed and replaced with sterile PBS and after three minutes it was replaced with trypsin-EDTA solution and then incubated at 37°C for 5 minutes. Complete medium (MEM with 10% FBS) was added to inactivate the trypsin, and centrifuged at 1500 rpm for 5 minutes. Cell pellet was resuspended in cryopreservation medium (80% FBS, 10% dimethylsulfoxide and 10% MEM medium) with a final concentration of 5 million cells per milliliter and aliquoted into cryovials. The vials stored at −80 °C overnight and then transferred into a liquid nitrogen container for long-term storage.