Cells will be placed on the printed patterns described above. Cells grown on discreet arrays will be fixed and stained for ALP activity (Sigma) after 1, 3, or 7 days in culture on the growth factor patterns. Mineralization will be tested after culturing cells on patterns in OM for 7, 14, or 21 days using alizarin red staining (for calcium) and von Kossa staining (for phosphates). ALP staining will be performed to identify early osteogenic differentiation. To signify "end" of BMP2 signaling, matrix mineralization will be determined by positive von Kossa or alizarin red staining using established protocols.