Equilibrium between the soil and the pore-water phase should ideally be established before adding the organisms; a time period of four days at 20 °C is recommended.
Ideaaljuhul tuleks enne organismide lisamist kindlaks teha, et mulla- ja pooriveefaasi vahel on tekkinud tasakaaluolek; soovituslik on neljapäevane ajavahemik 20 °C juures.