Measure the theoretical plate count of standard 1 (8.1) regularly and replace the column (6.1) when the plate count is 25 % lower than that of the initial value of a new column.
Mõõta regulaarselt standardi 1 (8.1) teoreetiliste taldrikute arvu ja asendada kolonn (6.1) siis, kui taldrikute arv on 25 % madalam uue kolonni vastavast algväärtusest.