N2 + 16ATP + 8e- + 8H+ -> 2NH3 + H2 + 16ADP + 16PiUsoro a jikọtara ya na hydrolysis nke ihe ruru 16 nke ATP ma soro ya na nhazi nke otu H2. Mgbanwe nke N2 n'ime amonia na-apụta na igwe igwe a na-akpọ FeMoco, nkenke maka ígwè-molybdenum cofactor. Usoro a na-aga n'ihu site na usoro nke protonation na mbelata ebe ebe ebe FeMoco nọ n'ọrụ na-eme ka N2 dị na mmiri. N'ime dioprophs na-ebi ndụ n'efu, a na-etinye amonia na-emepụta nitrogen n'ime glutamate site na glutamine synthetase / glutamatase.
ọnọdụ anaerobic