N'ime ule RT-PCR, a na-ewepụta RNA nje site na plasma onye ọrịa ma jiri reverse transcriptase (RT) mee ihe iji gbanwee RNA nje n'ime cDNA. A na-eji usoro mmeghachi omume nke polymerase (PCR) mee ihe, na-eji ihe abụọ dị iche iche na genome nke nje ahụ. Mgbe mmụba PCR zuru ezu, a na-eme ka ngwaahịa DNA pụta na oligonucleotides akọwapụtara na mgbidi arịa ahụ, wee mee ka a hụ ya na nyocha nke ejikọtara na enzyme. Enwere ike ịkọwapụta oke nje na nlele ahụ na izi ezi zuru oke iji chọpụta mgbanwe atọ. Ajụjụ: Kedu enzyme a na-eji na RT-PCR ule?
reverse transcriptase