Rịba ama amaokwu a dị n'okpuru ma zaa ajụjụ ahụ mgbe e mesịrị n'otu asụsụ ahụ dị ka amaokwu ahụ:  Usoro: N2 + 16ATP + 8e- + 8H+ -> 2NH3 + H2 + 16ADP + 16PiUsoro a jikọtara ya na hydrolysis nke ihe ruru 16 nke ATP ma soro ya na nhazi nke otu ihe ruru H2. A na-agbanwe N2 n'ime amonia na ụyọkọ ọla a na-akpọ FeMoco, nkenke maka iron-molybdenum cofactor. Usoro a na-aga n'ihu site na usoro nke protonation na mbelata ebe ebe ọrụ FeMoco na-eme ka N2 substrate dị. Na diazotrophs na-ebi ndụ n'efu, amonia nke nitrogenase mepụtara na-etinye aka na glutamate site na glutamine synthetase / glutamate synthase ụzọ. A na-ekesa mkpụrụ ndụ ihe nketa microbial nif dị mkpa maka idobe nitrogen n'ọtụtụ ebe na gburugburu ebe dị iche iche. Nitrogenases na-agbaji ngwa ngwa site na oxygen. N'ihi nke a, ọtụtụ nje bacteria na-akwụsị imepụta enzyme ahụ mgbe ikuku oxygen dị. Ọtụtụ ihe ndị dị ndụ na-ejikọta nitrogen na-adị nanị n'ọnọdụ anaerobic, na-eku ume iji belata ọkwa oxygen, ma ọ bụ na-ejikọta oxygen na protein dị ka leghemoglobin.  Ajụjụ: Ebee ka ihe ka ọtụtụ n'ụmụ anụmanụ ndị na-edozi nitrogen dị?
ọnọdụ anaerobic