 Hence, the observed value equals the linear sum

By fitting the observations of formula_41 under various solution conditions to this functional form, one can estimate formula_42 and formula_43, as well as the parameters of formula_31.The fitting variables formula_42 and formula_43 are sometimes allowed to vary linearly with the solution conditions, e.g., temperature or denaturant concentration, when the asymptotes of formula_41 are observed to vary linearly under strongly folding or strongly unfolding conditions.Assuming a two state denaturation as stated above, one can derive the fundamental thermodynamic parameters namely, formula_52, formula_53 and formula_31 provided one has knowledge on the formula_55 of the system under investigation.