 In practice, the observed experimental data at different denaturant concentrations are fit to a two-state model with this functional form for formula_31, together with linear baselines for the folded and unfolded states.The formula_28 and formula_29 are two fitting parameters, along with four others for the linear baselines (slope and intercept for each line); in some cases, the slopes are assumed to be zero, giving four fitting parameters in total.The conformational stability formula_31 can be calculated for any denaturant concentration (including the stability at zero denaturant) from the fitted parameters formula_28 and formula_29.