 Resultant map

Rapid Denaturation and Renaturation of a crude DNA preparation by alkaline lysis of the cells and subsequent neutralization

In this technique the cells are lysed in alkaline conditions.The DNA in the mixture is denatured (strands separated) by disrupting the hydrogen bonds between the two strands.The large genomic DNA is subject to tangling and staying denatured when the pH is lowered during the neutralization.