 It consists of two cells which are enclosed in an adiabatic jacket.The compounds to be studied are placed in the sample cell, while the other cell, the reference cell, is used as a control and contains the buffer in which the sample is dissolved.ITC is a quantitative technique that can determine the binding affinity (formula_1), enthalpy changes (formula_2), and binding stoichiometry (formula_3) of the interaction between two or more molecules in solution.