 On October 28, 1858, Barstow married Laura Maeck (1831–1885) of Shelburne.They were the parents of two sons, Frederick Maeck Barstow (1860–1899) and Charles Lester Barstow (1867–1951).Frederick Barstow graduated from the University of Vermont, worked as a civil engineer, and volunteered for service in the Spanish–American War.