 For example, if equal volumes of chloroform (water solubility 0.8 g/100 ml at 20 °C) and water are shaken together and then left to stand, the liquid will separate into two layers.Analysis of the layers shows that the top layer is mostly water with a small amount of chloroform dissolved in it, and the bottom layer is mostly chloroform with a small amount of water dissolved in it.If the two layers are heated together, the system of layers will boil at 53.3 °C, which is lower than either the boiling point of chloroform (61.2 °C) or the boiling point of water (100 °C).