 Among his portraits, he painted a portrait of Giovanni Guidiccione, bishop of Fossombrone; of Giulio dalla Rovere, Cardinal of Urbino, who died 1577; as well as Alessandro Sforza, Cardinal Legate of Romagna; Cristoforo Boncòmpagni, archbishop of Ravenna; and Giovanni Battista Rossi, Generale del Carmine, who died in Rome in 1577.His works include "The Marriage of Saint Catherine" (1529), "Lady and the Unicorn", "Adoration by the Shepherds", "Virgin and Child with Saints Sebastian and Rocco", "Martyrdom of Saint Ursicinus" (oil on canvas) and "Cesare Hercolani".He trained two of his children, Francesco Longhi (1544–1618) and Barbara Longhi (1552–1638).