 On January 13, 1835 Holbrook married to Harriet Goodhue, daughter of Joseph and Sarah Goodhue of Brattleboro.They were the parents of Franklin F. Holbrook, 
William C. Holbrook, and John Holbrook.The Deacon John Holbrook House built on Linden Street in Brattleboro by Holbrook's father still stands and is on the National Register of Historic Places.