 "De novo" DNA synthesis refers to the synthetic creation of DNA rather than assembly or modification of natural precursor template DNA sequences.Initial oligonucleotide synthesis is followed by artificial gene synthesis, and finally by a process cloning, error correction, and verification, which often involves cloning the genes into plasmids into Escherichia coli or yeast.Primase is an RNA polymerase, and it can add a primer to an existing strand awaiting replication.