 The enzyme was not activated by divalent cations nor inhibited by chelating agents.The determined Km value was 0.55mM and the pH optimum for the enzyme between 6.5 and 7.5.Found in different microorganisms such as Pseudomonas, Acetobacter, C. glutamicum, Veillonella parvula, and A. vinelandii, cytoplasmic oxaloacetate decarboxylases are dependent on the presence of divalent cations such as Mn2+, Co2+, Mg2+, Ni2+, or Ca2+.