 Timoner was born in Miami, Florida to Elissa and Eli Timoner, co-founder of Air Florida, in 1972.She has two siblings, Rabbi Rachel Timoner and David Timoner, who co-founded Interloper Films and has collaborated on several of her films.Timoner attended Yale University, where she founded the Yale Street Theater Troupe, a guerrilla theater ensemble that performed spontaneously in unexpected environments, in 1992.