 Biopsy specimens for DIF should be taken from perilesional skin instead of lesional skin for H&E histopathologic evaluation.DIF of bullous pemphigoid will show the presence of fine, continuous and linear deposits of IgG and/or C3 along the epidermal basement membrane.Other classes of immunoglobulins such as IgM and IgA are present in approximately 20% of cases and usually are less intense.