 Among all, direct immunofluorescence is the gold standard for diagnosis of mucous membrane pemphigoid.Lesional tissue, preferably of an intact vesicle or the edge of an intact bulla is obtained using punch biopsy for Haemotoxylin and Eosin (H&E) staining.The findings are sub-epidermal blister with dermal infiltrated with lymphocytes, neutrophils and eosinophils.