 The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography, since the polarity of the mobile and stationary phases have been inverted – hence the term reversed-phase chromatography.Reversed-phase chromatography employs a polar (aqueous) mobile phase.As a result, hydrophobic molecules in the polar mobile phase tend to adsorb to the hydrophobic stationary phase, and hydro"philic" molecules in the mobile phase will pass through the column and are eluted first.