 Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent, which reduces hydrophobic interactions.The more hydrophobic the molecule, the more strongly it will bind to the stationary phase, and the higher the concentration of organic solvent that will be required to elute the molecule.Many of the mathematical and experimental considerations used in other chromatographic methods also apply to RPC (for example, the separation resolution is dependent on the length of the column).