 This has been specifically used in the investigation of skeletal muscle development and the identification of its regulatory network.Systems approach using high-throughput sequencing and ChIP-chip analysis has been essential in elucidating the targets of myogenic regulatory factors like MyoD and myogenin, their inter-related targets, and how MyoD acts to alter the epigenome in myoblasts and myotubes.This has also revealed the significance of PAX3 in myogenesis, and that it ensures the survival of myogenic progenitors.