 It had also been used to study biofilm tolerance to antibiotics to differentiate tolerant and sensitive subpopulations 

Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available.Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4).The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids.