 Phylogenetic analysis shows that the southern population was derived from Northern Kyushu area and spread into Honshu island.On the other hand, Northern population was derived from Tango and spread alongside with Japan sea coast.Orizias Latipes is known to have nine sub populations, East Japanese type, East Setouchi type, West Setouchi type, Saninn type, Northern Kyusyu type, Osumi Type, Ariake type, Satuma type and Ryukyu type.