 The hormone vasopressin, stimulates the activity of NKCC2.Vasopressin stimulates sodium chloride reabsorption in the thick ascending limb of the nephron by activating signaling pathways.Vasopressin increases the traffic of NKCC2 to the membrane and phosphorylates some serine and threonine sites on the cytoplasmic N-terminal of the NKCC2 located in the membrane, increasing its activity.