 Lipidomic studies play an essential role in defining the biochemical mechanisms of lipid-related disease processes through identifying alterations in cellular lipid metabolism, trafficking and homeostasis.The two major platforms currently used for lipidomic analyses are HPLC-MS and shotgun lipidomics.Shotgun lipidomics was developed by Richard W. Gross and Xianlin Han, by employing ESI intrasource separation techniques.