 Glenrothes' greatest success as a club came in 1975 when they won the Scottish Junior Cup, defeating Rutherglen Glencairn 1–0, in front of a crowd of 17,776 at Hampden Park.Glens had also been runners-up in 1968, losing after a replay to Johnstone Burgh with a combined attendance of 50,000 over the two ties, an impressive achievement given that they had only been formed a mere four years beforehand.The club were members of the Fife Junior League, before competing in the East Region of which they, along with all Fife junior clubs became a part of in 2002.