 RF3 is still needed to release RF1/2 from this translation termination complex.After releasing the peptide, ribosomal recycling is still required to empty the P-site tRNA and mRNA out to make the ribosome usable again.This is done by splitting the ribosome with factors like IF1–IF3 or RRF–EF-G.

eRF1 can be broken down into four domains