 The DMEM usually contains 1.5 g/L sodium bicarbonate, 2 mM L-Glutamine, 1 mM sodium pyruvate and 0.1 mM nonessential amino acids.The cells are always grown at 37 degrees Celsius with 95% air and 5% carbon dioxide.It is advised to cultivate the cells in flasks which are coated for cell culture adhesion, this will aid in differention and dendrification of the neuroblastoma.