 A neuroblast-like subclone of SK-N-SH, named SH-SY, was subcloned as SH-SY5, which was subcloned a third time to produce the SH-SY5Y line, first described in 1978.The cloning process involved the selection of individual cells or clusters expressing neuron-like characteristics.The SH-SY5Y line is genetically female with two X and no Y chromosome, as expected given the origin from a four-year-old female.