 This turned out to be a shrewd move - he became a millionaire in a relatively short time and by the early 20th century was estimated to be worth $30 million.In 1901 he isolated and purified the hormone adrenaline (the first effective bronchodilator for asthma) from animal glands, becoming the first to accomplish this for a glandular hormone.In 1894, Takamine applied for, and was granted, a patent titled "Process of Making Diastatic Enzyme" ()—the first patent on a microbial enzyme in the United States.