sentences:  This approach provided the underlying methodology for next-generation sequencing analyses to detect chromosomal abnormalities in human cancer as well as in prenatal genetic testing.In parallel, Velculescu was an early developer of methods for high-throughput sequencing of human cancer, which his group used to identify the PIK3CA gene as one of the most highly mutated cancer genes.Starting in 2005, Velculescu extended these approaches, and together with Bert Vogelstein, Ken Kinzler and other colleagues at Johns Hopkins performed the first sequence analysis of the coding genome in human cancers, including breast, colorectal, brain, and pancreatic cancers.