sentences:  In older dogs that develop distemper encephalomyelitis, diagnosis may be more difficult, since many of these dogs have an adequate vaccination history.An additional test to confirm distemper is a brush border slide of the bladder transitional epithelium of the inside lining from the bladder, stained with Diff-Quik.These infected cells have inclusions which stain a carmine red color, found in the paranuclear cytoplasm readability.