sentences:  Dark discoloration of vascular bundles is visible shortly thereafter.Once inside the vascular tissue, "R. bunodes" compromises xylem flow and transport of nutrients to aerial parts of the host resulting in chlorosis, wilting, dry die-back, leaf drop, and eventually host death.In controlled laboratory experiments, vegetative symptoms like chlorosis and wilting in two-year-old poplar ("Populus deltoides") saplings were observed 14 days after inoculation, and field observations describe death of mature trees within 3–4 years.