sentences:  The "FLCN" gene encodes a 64 kDa protein, FLCN, which is highly conserved across species.The majority of germline "FLCN" mutations identified in BHD patients are loss-of-function mutations including frameshift mutations (insertion/deletion), nonsense mutations, and splice site mutations that are predicted to inactivate the FLCN protein, although some missense mutations have been reported that exchange one nucleotide for another and consequently result in a different amino acid at the mutation site.Most mutations are identified by DNA sequencing.