sentences:  FLCN has been shown to interact through its C-terminus with two novel proteins, folliculin interacting protein 1 (FNIP1) and folliculin interacting protein 2 (FNIP2/FNIPL), and indirectly through FNIP1 and FNIP2 with AMP-activated protein kinase (AMPK).AMPK is an important energy sensor in cells and negative regulator of mechanistic target of rapamycin (mTOR)  suggesting that FLCN and FNIP1 may play a role in modulating mTOR activity through energy or nutrient sensing pathways.Coimmunoprecipitation experiments with FNIP1 and FLCN expressed in HEK293 cells and in vitro binding assays have shown that the C-terminus of FLCN and amino acids 300 to 1166 of FNIP1 are required for optimal FLCN-FNIP1 binding.