sentences:  Polymerase Cycling Assembly and TAR technology were used together to construct the 600 kb "Mycoplasma genitalium" genome in 2008, the first synthetic organism ever created.Similar steps were taken in synthesizing the larger "Mycoplasma mycoides" genome a few years later.An unnatural base pair (UBP) is a designed subunit (or nucleobase) of DNA which is created in a laboratory and does not occur in nature.