sentences:  For example, it binds human VκI, VκIII and VκIV subtypes but does not bind the VκII subtype.Binding of mouse immunoglobulins is restricted to those having VκI light chains.Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain.