sentences:  Once the tubulins are in contact with the chaperonin, the prefoldin automatically lets go and leaves the active site, due to its high affinity for the chaperonin molecule.Once the prefoldin is in contact with the chaperonin protein, it loses its affinity for the unfolded target protein.Prefoldin is triggered only to bind to nonnative target proteins in the cytosol so that it will only bind to unfolded proteins.