sentences:  Some of the insertions affected the functions of MBP whereas others were permissive.The permissive sites that were internal to MBP, were used to insert antigenic peptides and challenge the immune response in mice.The 3'-OH terminal insertions were used to create fusion proteins and develop the use of MBP as an affinity handle for the purification of foreign proteins and peptides by affinity chromatography on cross-linked amylose and elution with maltose in mild physico-chemical conditions.