sentences:  Cdc45 targets the Mcm protein complex, which has been loaded onto the chromatin, as a component of the pre-RC at the origin of replication during the G1 stage of the cell cycle.Cell division cycle 45 (Cdc45) protein is a critical component for the conversion of the pre-replicative complex to the initiation complex.The Cdc45 protein assembles at replication origins before initiation and is required for replication to begin in "Saccharomyces cerevisiae", and has an essential role during elongation.