sentences:  After cleaving the fusion protein, the recombinant batroxobin could be isolated by electrophoresis and it was then successfully refolded to produce biologically active batroxobin.This study showed that it was possible to produce batroxobin using micro-organisms, a method which was more promising than isolating the enzyme from extracted snake venom.In 2004, a research group from Korea produced batroxobin by expressing it in the yeast species "Pichia pastoris".