sentences:  These origins of replication direct the number of protein complexes that will form to initiate replication.In prokaryotic DNA replication regulation focuses on the binding of the DnaA initiator protein to the DNA, with initiation of replication occurring multiple times during one cell cycle.Both prokaryotic and eukaryotic DNA use ATP binding and hydrolysis to direct helicase loading and in both cases the helicase is loaded in the inactive form.