sentences:  In "E. coli" these proteins include DiaA, SeqA, IciA, HU, and ArcA-P, but they vary across other bacterial species.A few other mechanisms in "E. coli" that variously regulate initiation are DDAH ("datA"-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the "dnaA" gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane.Once priming is complete, DNA polymerase III holoenzyme is loaded into the DNA and replication begins.