sentences:  For the same reason, the initiation of DNA replication is highly regulated.Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery.In "E. coli," the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication ("oriC") which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom).