sentences:  Geminin first appears in S-phase and is degraded at the metaphase-anaphase transition, possibly through ubiquination by anaphase promoting complex (APC).Various cell cycle checkpoints are present throughout the course of the cell cycle that determine whether a cell will progress through division entirely.Importantly in replication the G1, or restriction, checkpoint makes the determination of whether or not initiation of replication will begin or whether the cell will be placed in a resting stage known as G0.