sentences:  According to the Van´t Hoff equation, the relation between free energy, Δ"G", and "K" is Δ"G°" = -"RT"ln "K", where "R" is the ideal gas law constant, and "T" is the kelvin temperature of the reaction.This gives, for the nucleic acid system,
  
  formula_2.The melting temperature, "T"m, occurs when half of the double-stranded nucleic acid has dissociated.