sentences:  The free energy of forming this DNA from the individual strands, Δ"G"°, is represented (at 37 °C) as
  
  Δ"G"°37(predicted) = Δ"G"°37(C/G initiation) + Δ"G"°37(CG/GC) + Δ"G"°37(GT/CA) + Δ"G"°37(TT/AA) + Δ"G"°37(TG/AC) + Δ"G"°37(GA/CT) + Δ"G"°37(A/T initiation)
  
  Except for the C/G initiation term, the first term represents the free energy of the first base pair, CG, in the absence of a nearest neighbor.The second term includes both the free energy of formation of the second base pair, GC, and stacking interaction between this base pair and the previous base pair.The remaining terms are similarly defined.