sentences:  Based on the finding of different transition state structures, Schramm and coworkers designed two transition state analogues mimicking the early and late dissociative transition state.The early and late transition state analogue shown binding affinity (Kd) of 360 and 140 pM, respectively.Thermolysin is an enzyme produced by "Bacillus thermoproteolyticus" that catalyses the hydrolysis of peptides containing hydrophobic amino acids.