sentences:  Because of the relative simplicity, one of the most used methods is using biotinylated reverse primers in the amplification step, after which the complementary strands can be bound to a resin followed by elution of the other strand with lye.Another method is asymmetric PCR, where the amplification step is performed with an excess of forward primer and very little reverse primer, which leads to the production of more of the desired strand.A drawback of this method is that the product should be purified from double stranded DNA (dsDNA) and other left-over material from the PCR reaction.