sentences:  The first reported glycosynthase was a mutant of the Agrobacterium sp.β-glucosidase / galactosidase in which the nucleophile glutamate 358 was mutated to an alanine by site directed mutagenesis.When incubated with α-glycosyl fluorides and an acceptor sugar it was found to catalyze the transglycosidation reaction without any hydrolysis.