Sure thing! We've got 453109 and 77564, and we're going to subtract the second number from the first.

Step 1: We'll start by subtracting the digit 4 and the borrow 0 from 9 in column 1 and get 5.
5 is the first digit of our result.

Step 2: We'll start by subtracting the digit 6 and the borrow 0 from 0 in column 2 and get -6.
We add -6 to 10 and get 4 as the next digit of the result.

Step 3: We'll start by subtracting the digit 5 and the borrow 1 from 1 in column 3 and get -5.
We add -5 to 10 and get 5 as the next digit of the result.

Step 4: We'll start by subtracting the digit 7 and the borrow 1 from 3 in column 4 and get -5.
We add -5 to 10 and get 5 as the next digit of the result.

Step 5: We'll start by subtracting the digit 7 and the borrow 1 from 5 in column 5 and get -3.
We add -3 to 10 and get 7 as the next digit of the result.

Step 6: We'll start by subtracting the digit 0 and the borrow 1 from 4 in column 6 and get 3.
3 is the next digit of our result.

So, 453109 - 77564 = 375545.