Let's dive into this subtraction. We'll start with 65385 and subtract 35976 from it.

Step 1: We'll start by subtracting the digit 6 and the borrow 0 from 5 in column 1 and get -1.
We add -1 to 10 and get 9 as the first digit of the result.

Step 2: We'll start by subtracting the digit 7 and the borrow 1 from 8 in column 2 and get 0.
0 is the next digit of our result.

Step 3: We'll start by subtracting the digit 9 and the borrow 0 from 3 in column 3 and get -6.
We add -6 to 10 and get 4 as the next digit of the result.

Step 4: We'll start by subtracting the digit 5 and the borrow 1 from 5 in column 4 and get -1.
We add -1 to 10 and get 9 as the next digit of the result.

Step 5: We'll start by subtracting the digit 3 and the borrow 1 from 6 in column 5 and get 2.
2 is the next digit of our result.

So, 65385 - 35976 = 29409.