We can solve this together! We're beginning with 65429 and removing 43116 from it.

Step 1: We'll start by subtracting the digit 6 and the borrow 0 from 9 in column 1 and get 3.
3 is the first digit of our result.

Step 2: We'll start by subtracting the digit 1 and the borrow 0 from 2 in column 2 and get 1.
1 is the next digit of our result.

Step 3: We'll start by subtracting the digit 1 and the borrow 0 from 4 in column 3 and get 3.
3 is the next digit of our result.

Step 4: We'll start by subtracting the digit 3 and the borrow 0 from 5 in column 4 and get 2.
2 is the next digit of our result.

Step 5: We'll start by subtracting the digit 4 and the borrow 0 from 6 in column 5 and get 2.
2 is the next digit of our result.

So, 65429 - 43116 = 22313.