Sure thing! We've got 458386 and 36301, and we're going to subtract the second number from the first.

Step 1: We'll start by subtracting the digit 1 and the borrow 0 from 6 in column 1 and get 5.
5 is the first digit of our result.

Step 2: We'll start by subtracting the digit 0 and the borrow 0 from 8 in column 2 and get 8.
8 is the next digit of our result.

Step 3: We'll start by subtracting the digit 3 and the borrow 0 from 3 in column 3 and get 0.
0 is the next digit of our result.

Step 4: We'll start by subtracting the digit 6 and the borrow 0 from 8 in column 4 and get 2.
2 is the next digit of our result.

Step 5: We'll start by subtracting the digit 3 and the borrow 0 from 5 in column 5 and get 2.
2 is the next digit of our result.

Step 6: We'll start by subtracting the digit 0 and the borrow 0 from 4 in column 6 and get 4.
4 is the next digit of our result.

So, 458386 - 36301 = 422085.