No problem, let's work through this together. We're starting with 38453 and are subtracting 10109 from it.

Step 1: We'll start by subtracting the digit 9 and the borrow 0 from 3 in column 1 and get -6.
We add -6 to 10 and get 4 as the first digit of the result.

Step 2: We'll start by subtracting the digit 0 and the borrow 1 from 5 in column 2 and get 4.
4 is the next digit of our result.

Step 3: We'll start by subtracting the digit 1 and the borrow 0 from 4 in column 3 and get 3.
3 is the next digit of our result.

Step 4: We'll start by subtracting the digit 0 and the borrow 0 from 8 in column 4 and get 8.
8 is the next digit of our result.

Step 5: We'll start by subtracting the digit 1 and the borrow 0 from 3 in column 5 and get 2.
2 is the next digit of our result.

So, 38453 - 10109 = 28344.