Let's dive into this subtraction. We'll start with 99509 and subtract 65386 from it.

Step 1: We'll start by subtracting the digit 6 and the borrow 0 from 9 in column 1 and get 3.
3 is the first digit of our result.

Step 2: We'll start by subtracting the digit 8 and the borrow 0 from 0 in column 2 and get -8.
We add -8 to 10 and get 2 as the next digit of the result.

Step 3: We'll start by subtracting the digit 3 and the borrow 1 from 5 in column 3 and get 1.
1 is the next digit of our result.

Step 4: We'll start by subtracting the digit 5 and the borrow 0 from 9 in column 4 and get 4.
4 is the next digit of our result.

Step 5: We'll start by subtracting the digit 6 and the borrow 0 from 9 in column 5 and get 3.
3 is the next digit of our result.

So, 99509 - 65386 = 34123.