DSB
DSB may refer to:

Science and technology
 Dictionary of Scientific Biography, a multivolume reference work edited by Charles Coulston Gillespie
 Double strand break, a break in both DNA strands, part of DNA repair
 Double-sideband transmission, in telecommunications
 Dsb, the warm-summer Mediterranean continental climate in the Köppen climate classification
 DsbA, a bacterial member of the Dsb (disulfide bond) family of enzymes

Organizations
 De Surinaamsche Bank, Suriname
 Defense Science Board, of the United States
 Department of Internal Security (:ru:Главное управление собственной безопасности МВД России) or Departamenta sobstvennoi bezopasnosti of the Russian Ministry of Internal Affairs, an anticorruption and internal crime detection agency in Russia
 Deutsche Schallplatten Berlin, another name of VEB Deutsche Schallplatten
 Deutsche Schule Bratislava, a German international school in Bratislava, Slovakia
 Deutscher Schützenbund, the German Shooting and Archery Federation
 Dispute Settlement Body, of the World Trade Organization
 DSB (railway company) (Danske Statsbaner), a Danish train operating company
 DSB Bank (Dirk Scheringa Beheer), Netherlands
 DSB Stadion, a football stadium in Alkmaar, Netherlands sponsored by DSB Bank
 DSB International School, a German school in Mumbai, India
 Dutch Safety Board, a Netherlands investigation agency
 German International School of Cairo (Deutsche Schule der Borromäerinnen Kairo), Egypt
 German School Beirut (Deutsche Schule Beirut), Lebanon
 Norwegian Directorate for Civil Protection (Direktoratet for samfunnssikkerhet og beredskap), a Norwegian government organization

Other
 Dark square bishop, in chess
 Democrats for a Strong Bulgaria, a Bulgarian political party
 Department Store Battles, a modification for Battlefield 2, a computer game
 Domestic Stability Buffer, a capital buffer set by a financial regulator in Canada requiring major domestic banks to keep some loan funds in reserve
 Don Salvador Benedicto, a municipality in the province of Negros Occidental, Philippines
 "Don't Stop Believin'", a song by American rock band Journey
 DSB, the ISO 639-2 or SIL International language code for the Lower Sorbian language - Dolnoserbski