Mr. Universe may refer to:

Bodybuilding contests
 IFBB Mr. Universe, now the , a male bodybuilding contest organised by the International Federation of BodyBuilding & Fitness (IFBB)
 NABBA Mr. Universe, a Pro/Am competition at the Universe Championships annual bodybuilding event.

Entertainment
 Jim Gaffigan: Mr. Universe, 2012 stand-up special by comedian Jim Gaffigan
 Mr. Universe (album), a 1979 album by the British rock band Gillan
 Mister Universe (film), the 1951 film debut of Vince Edwards
 Mister Universo 2016 Austrian-Italian film in which 1957 Mr. Universe Arthur Robin plays himself
 Mr. Universe, a 1988 Hungarian feature film with a cameo by 1955 Mr. Universe Mickey Hargitay playing himself
 Mr. Universe, a character in the 2005 film Serenity
 Mr. Universe, the stage name of Greg Universe from Cartoon Network show Steven Universe