Queen Matilda may refer to:

 Matilda (877–968), Saint Matilda, Queen of Eastern Francia
 Matilda of Frisia (died 1044), Queen of the Franks, wife of King Henry I
 Matilda of Flanders (c. 1031–1083), Queen of the English, wife of William the Conqueror
 Maud of Northumbria (c. 1074–1130/1131), Queen of Scotland, wife of King David I
 Matilda of Scotland (c. 1080–1118, Queen of the English, wife of King Henry I
 Empress Matilda (c. 1102–1167), Matilda of England, Holy Roman Empress, Queen of Germany, claimant to throne of England
 Matilda I of Boulogne (c. 1105–1152), Queen of the English, wife of King Stephen
 Matilda of Savoy (1125–1158), Queen of Portugal, wife of King Afonso I
 Matilda II of Boulogne (1202–1259), Queen of Portugal, wife of King Afonso III
 Maud of Wales (1869–1938), Queen of Norway, wife of King Haakon VII
 Mathilde d'Udekem d'Acoz (born 1973), Queen of the Belgians, wife of King Philippe