The Fountain of Youth is a legendary spring said to restore youth.

Fountain of Youth can also refer to:

 Fountain of Youth (album), a 2014 album by The Rippingtons
Fountain of Youth (Cranach), a painting by Lucas Cranach the Elder
The Fountain of Youth (fairy tale), a Japanese fairy tale
The Fountain of Youth (film), a 1958 television pilot directed by Orson Welles
"Fountain of Youth" (The Mighty Boosh episode), an episode of the British comedy TV series The Mighty Boosh
Fountain of Youth (Wolfe), orchestral composition by Julia Wolfe
Fountain of Youth Archaeological Park, a tourist attraction in St. Augustine, Florida, United States
Fountain of Youth Stakes, a Thoroughbred race held in Florida
Don's Fountain of Youth, a 1953 Donald Duck short